3C (Chromatin Conformation Capture): A Technique to Study Chromatin Organization
نویسندگان
چکیده
منابع مشابه
Chromosome Conformation Capture (3C) (PROT05)
An alternative protocol (PROT31) for 3C has been adopted by the author's lab. Much of it is identical to this version. The major differences are in the amounts of DNA used at different steps. We reliably get the same results as with this version, but also a greater yield of 3C material. Yields from this version are ample for real-time PCR analysis, but greater yields are required if the 3C mate...
متن کامل05-P013 Analysis of chromatin organization of Hox gene cluster by using 3C-based technique
tors, contribute to the specification of neuronal subtype identities, and promote neuronal migration and differentiation. Little is known, however, on the molecular mechanisms underlying the gene regulatory functions of proneural factors. The identity of their target genes, and how do they co-ordinately regulate the neurogenic program is not known. These issues cannot be addressed in the mouse ...
متن کاملMapping chromatin conformation
Chromatin conformation capture (3C) and related techniques have become well-established methods to examine which distal DNA sequences are spatially located close to a locus of interest. Hi-C is a new addition to the growing set of 3C-based techniques that has extended the approach to a genome-wide analysis of nuclear chromatin using high-throughput DNA sequencing to generate global interaction ...
متن کاملChromatin Organization
Although the Victorian proverb ‘‘everything in its place and a place for everything’’ is meant for orderly households, it could just as easily describe the organization of eukaryotic genomes. This issue’s Select profiles articles that reveal new insights into how DNA is packaged and partitioned into three-dimensional (3D) structures throughout the life of a cell, shedding new light on how inter...
متن کاملChromosome Conformation Capture (3C) in Budding Yeast.
Chromosome conformation capture (3C) is a method for studying chromosomal organization that takes advantage of formaldehyde cross-linking to measure the spatial association of two pieces of chromatin. The 3C method begins with whole-cell formaldehyde fixation of chromatin. After cell lysis, solubilized chromatin is digested with a type II restriction endonuclease, and cross-linked DNA fragments...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Life Science
سال: 2012
ISSN: 1225-9918
DOI: 10.5352/jls.2012.22.11.1587